Earticle

초록

영어

Thermoplasma acidophilum is a thermoacidophilic archaeon that thrives aerobically at 59˚C and pH 2. The genome of T. acidophilum contains 1,564,905 bp on a single chromosome and encodes 1,509 ORFs, including a complete protein degradation pathway and various transport proteins1). Unfortunately, however, as much as 45% of its genome was annotated as hypothetical proteins. Hereby, we investigated how many intracellular proteins are expressed in
D-glucose medium by means of 2D gel electrophoresis and gel-free shotgun proteomics. Among more than 300 spots visualized in 2D gels, 190 spots could be analyzed by MALDI-TOF MS/MS. Additionally, 381 different proteins were identified by gel-free LC-MS/MS. When the generated data were searched using MASCOT and Phenyx database, we were able to identify 470 different
proteins in total. When identified proteins are divided into subgroups with regard to their functional categories, energy production and conversion is the largest group occupying 23% protein. Interestingly, 53% of the identified proteins have been detected at more than one position on the 2D electrophoresis gels, which suggests that these proteins are subject to regulation by PTMs.

저자

• Suk Min Kim [ Department of Chemical Engineering ]
• Sun Bok Lee [ Department of Chemical Engineering, School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576