Redesign of the existing natural biological systems requires many components that can be controlled by purpose. Protein functions as well as regulation mechanisms, first of all, should be understood in a systematic manner for well-controlled system. Although many methods are already used to clarify protein functions, they have a limitation on the high-throughput analysis by reason of the labor-intensive and time-consuming process. In this study, we developed a rapid and simple method to analyze protein functions efficiently based on the PCR-based site-directed mutagenesis and the expressional PCR using a coupled in vitro transcription/translation system derived from E. coli and eGFP (enhanced green fluorescence protein) gene as a template.1-2) Various deletion mutants showed different fluorescence activity. The results also showed that this method allows a rapid and simple route for the functional genetics.
저자
Sang Woo Seo [ Department of Chemical Engineering ]
Jin A Yang [ Department of Chemical Engineering ]
Gyoo Yeol Jung [ Department of Chemical Engineering, I-Bio Program, POSTECH ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동