Earticle

초록

영어

Outbreak of antibiotics resistant bacteria is a serious concern to medical, pharmaceutical, and food industry. While there are many accumulating evidences and speculations on how bacteria develop resistance to antibiotics, systems-level cataloging of potential resistance mechanisms has not been done. In this study, we attempted to identify putative gene targets that induce antibiotics resistance using a systematic knockout collection. Specifically, an E. coli knockout library based on the Keio collection was challenged with various concentrations of
ampicillin in order to isolate ampicillin resistant knockout mutants. Of 48 colonies grown on various ampicillin concentrations, seven knockout mutants exhibiting significant resistance were isolated. To confirm that the resistance phenotype was caused not by spontaneous mutations but by a known single gene mutation, the mutations (single gene knockout) from the seven mutants were transferred into E. coli DH5α strain having a different genotypic background by P1 transduction. All of the identified single gene knockouts conferred ampicillin resistance into the E. coli DH5α

키워드

저자

• Min-Eui Hong [ Department of Food Science & Biotechnology, Sungkyunkwan University, Suwon, Korea ]
• Jieun Lee [ Department of Food Science & Biotechnology, Sungkyunkwan University, Suwon, Korea ]
• Yong-Su Jin [ Department of Food Science & Biotechnology, Sungkyunkwan University, Suwon, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576