Yu-Sin Jang, Seh Hee Jang, Hyohak Song, Sang Yup Lee
언어
영어(ENG)
URL
https://www.earticle.net/Article/A100218
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원문정보
초록
영어
Recent studies have raised industrial application of Mannheimia succiniciproducens to produce succinic acid.1) Shuttle vectors replicating in E. coli and M. succiniciproducens were constructed. These vectors were present at ca. 10 copies per cell and stably maintained in M. succiniciproducens during the serial subculturing without antibiotic for 48 generations. By optimizing the electroporation conditions, the transformation efficiencies of 3.0×106 transformants/mg DNA were obtained in M.succiniciproducens. In addition, there were compatible restriction and modification systems judged from the transformation efficiencies in E. coli K strain and M. succiniciproducens. DNA fragments up to 8.2 kb in size can be cloned into shuttle vectors. Thus, the shuttle vectors developed in this study are useful tools for genetic and metabolic engineering of rumen bacteria. (This work was supported by the Genome-based Integrated Bioprocess Development Project (No. 2005-01294) of the Ministry of Science and Technology through the Korea Science and Engineering Foundation (KOSEF). Further supports by the LG Chem Chair Professorship, IBM SUR program, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.)
저자
Yu-Sin Jang [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical &Biomolecular Engineering (BK21 program) and BioProcess Engineering Research Center ]
Seh Hee Jang [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical &Biomolecular Engineering (BK21 program) and BioProcess Engineering Research Center ]
Hyohak Song [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical &Biomolecular Engineering (BK21 program) and BioProcess Engineering Research Center ]
Sang Yup Lee [ Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical &Biomolecular Engineering (BK21 program) and BioProcess Engineering Research Center, Department of BioSystems and Bioinformatics Research Center, Korea Advanced Institute of Science and Technology ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동