Seung Hee Woo, Mi Ran Park, Min Jeong Hwang, Hye Jin Choi, Seon A Kim, Hye Jeong Choi, Hyung Chul Park, Jeong Uck Park, Yong Kee Jeong, Dong Wan Kim, Woo Hong Joo
언어
영어(ENG)
URL
https://www.earticle.net/Article/A100216
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원문정보
초록
영어
The Pseudomonas strain conserves a defense mechanism for the oxidative stress including the superoxide dismutase and catalase systems1-4). The genes for the defense mechanism, sodB and katA from an organic solvent tolerant Pseudomonas isolate were synthesized by PCR and cloned into pGEM-T easy vector for phylogenetic analysis. As a result, the sodB sequence showed more than 80% identity, in comparison with that of the Aeromonas species including A. bestiarum, A. hydrophila, A. bestiarum, A. media, and A. bestiarum, respectively. The nucleotide sequence of the sodB was also significantly homologous to that of P. putida, P. entomophila, P. fluorescens, P. aeruginosa, P. mendocina, and P. syringae, respectively. The katA sequence from the isolate was, of note, highly homologous to the catalase gene sequence of Aeromonas hydrophila subsp. Hydrophila, scoring 90% identity. This finding may give a clue to the significant phylogenetic relationship among the sodB sequence of Pseudomonas putida and Aeromonas species. High level expression and purification of the protein, superoxide dismutase B using the expression vector system is under the study.
저자
Seung Hee Woo [ Department of Biology, Changwon National University ]
Mi Ran Park [ Department of Biology, Changwon National University ]
Min Jeong Hwang [ Department of Biology, Changwon National University ]
Hye Jin Choi [ Department of Biology, Changwon National University ]
Seon A Kim [ Department of Biology, Changwon National University ]
Hye Jeong Choi [ Department of Biology, Changwon National University ]
Hyung Chul Park [ Department of Biology, Changwon National University ]
Jeong Uck Park [ Department of Biology, Changwon National University ]
Yong Kee Jeong [ Department of Biotechnology, Faculty of Natural Resources and Life Science, Dong-A University ]
Dong Wan Kim [ Department of Microbiology, Changwon National University ]
Woo Hong Joo [ Department of Biology, Changwon National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동