In the present study, we performed a differential proteomic analysisusing two-dimensional gel electrophoresis (2-DE) combined with mass spectrometry to clarify the molecular mechanism for suppressive effect of chitosan oligosaccharide (CO) during differentiation of adipocyte 3T3-L1.1),2) The cell differentiation was significantly inhibited by CO at the concentration of 4 mg/mL. The protein mapping of adipocyte homogenates by 2-DE revealed that numerous protein spots were differentially altered in response to CO treatment. Out of 50 identified proteins showing significant alterations, 6 were up-regulated and 44 were down-regulated by CO treatment in comparison to control mature adipocytes. Among them, most of the proteins are associated with lipid metabolism, cytoskeleton, and redox regulation. Taken together with immunoblot analysis, it was concluded that the inhibitory effect of CO on adipocyte differentiation was mediated by C/EBPα and PPARγ pathway through significant down-regulations of important adipogenic molecules such as fatty acid binding protein and glucose transporter 4.
저자
Md. Atiar Rahman [ Department of Biotechnology,Daegu University ]
Suresh G Kumar [ Department of Biotechnology,Daegu University ]
Jong Won Yun [ Department of Biotechnology,Daegu University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동