Earticle

초록

영어

The aim of this study was to characterize global changes of oxidative stress proteins induced by capsaicin in human hepatocarcinoma (HepG2) and human neuroblastoma cells (SK-N-SH) so as to clarify the mechanism of capsaicin-induced apoptosis. For this aim, we first investigated the effect of capsaicin on the induction of apoptosis using MTT assay and ROS levels during apoptosis. Capsaicin (200 µM) exhibited significant inhibitory effects against these two cell lines to about 50% after 24 h treatment. Interestingly, the ROS levels were increased during HepG2 apoptosis, whereas decreased in the course of SK-N-SH apoptosis in response to capsaicin treatments. Next, to investigate the mechanism of both cell apoptosis induced by capsaicin, we used proteomics to analyze the altered protein expression in both cells, with a special attention on oxidative stress proteins before and after capsaicin treatments. The 2-DE analysis demonstrated that 260 spots from about 1,500 visualized spots in HepG2 cells and 220 spots from 1,050 visualized spots were differentially regulated in SK-N-SH cells. Among the differentially expressed proteins, 23 proteins were significantly increased and 26 proteins were markedly decreased in capsaicin-treated HepG2 and SK-N-SH cells, respectively. The identified differential proteins appear to be involved in mainly oxidative stress, cancer, and apoptosis. The distinct effect of capsaicin-induced apoptosis on the expression pattern of HepG2 proteins includes the down-regulation of some antioxidant enzymes including aldose reductase, catalase, enolase 1, peroxiredoxin 1, but up-regulation of peroxiredoxin 6, cytochrome c oxidase, and SOD2. In contrast, most antioxidant enzymes were increased in SK-N-SH cells in response to capsaicin, where SOD2 might play a pivotal role in maintenance of low ROS levels in the course of apoptosis. The global gene expression for oxidative stress and antioxidant genes was analyzed by the Human Oxidative Stress and Antioxidant Defense RT² Profiler™ PCR Array. Surprisingly, the profiles of 84 gene expressions related to oxidative stress were not significantly different in HepG2 cells between control and capsaicin-treated cells, whereas alterations were very significant in SK-N-SH cells suggesting that those genes responded significantly to capsaicin treatment, thereby leading to low ROS levels during apoptosis.

저자

• Yu Mi Baek [ Dept. of Biotechnology, Daegu University ]
• Hye Jin Hwang [ Dept. of Biotechnology, Daegu University ]
• Jong Won Yun [ Dept. of Biotechnology, Daegu University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576