Earticle

초록

영어

Racemase is novel enzyme which has been biochemically classified as a subgroup of the isomerases. Although many report of sulfur containing amino acids transformation have been studied, homocysteine and sulfur containing amino acid racemase was not clearly identified. We here report the identification of free homocysteine isomers without making any derivatization. The separation of homocysteine isomers were performed on a chiralpacR MA(+) column by isocratic elution with 2mM copper sulfate-acetonitrile (85:15, v/v) as mobile phase. Authentic amino acid, D or L-homocysteine was incubated with crude extracts of Pseudomonas putida and diminuta under the neutral conditions at 37 ℃ for 3 hours. The change of proportion for D and L- isomers were detected and analyzed by UV-visible detector at 240nm within 30 minutes. Our new detection method for homocysteine isomers demonstrates the high sensitivity and rapid separation of each isomer and enabled us to detect the putative homocysteine racemase. The Pseudomonas putida exhibits a more great potential for preparative bio-transformations than diminuta. [Supported by grant No. R01-2006-000-10232-0 from MOST]

저자

• Hyun-Nam Cho [ Department of Applied Chemistry, Kumoh National Institute of Technology ]
• Dong-Chan Yoo [ Department of Applied Chemistry, Kumoh National Institute of Technology ]
• Bo-Eun Kim [ Department of Applied Chemistry, Kumoh National Institute of Technology ]
• Lu-An Fan [ Department of Applied Chemistry, Kumoh National Institute of Technology ]
• Kwang-Hwan Jhee [ Department of Applied Chemistry, Kumoh National Institute of Technology ]

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• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576