Earticle

초록

영어

PEI used as an effective non-viral gene delivery vector in vitro and in vivo1). Although it has high transfection efficiency, it makes a damage to normal cell membrane because of its low targeting efficiency to target cell or tissue2,3). In order to overcome the problem, vitamin as a targeting moiety was introduced into PEI. Vitamin can act as a ligand for targeting of nucleus membrane4). The conjugation of PEI/vitamin were confirmed by UV-spectrophotometer and
TNBSmethod, and characterized by gel electrophoresis, particle size and zeta potential to estimate a possibility as a gene carrier. In the case of PRA/DNA complex, a completed complexation was observed above 3 of N/P ratio, while PEI/DNA complex without PRA showed above 2. The size was appropriate to the cell transfection. The zeta potential of the conjugates decreased with increasing vitamin concentration. The transfection efficiency of PRA in HeLa cell
was up to 10 times higher than that of PEI only. The cytotoxicity of PRA was also measured by MTT assay. The conjugates showed lower cytotoxicity compared with the cationic polymer system. Therefore, the PRA conjugate may be good candidate for non-viral gene carrier.

저자

• Kyong Mi Park [ Department of Biotechnology, The Catholic University of Korea ]
• Kun Na [ Department of Biotechnology, The Catholic University of Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576