Earticle

초록

영어

At present, cell-based drug screening assays have been attracted attention for various molecular interactions including induction of apoptosis. Apoptosis is the process of programmed cell death through a tightly controlled program that plays important roles in many normal processes, ranging from fetal development to adult tissue homeostasis.1) Screening of apoptosis would allow
both early detection of therapy efficiency and evaluation of disease progression.2) In this study, the affinity of specific binding between annexin V and liposome that consists of several molar ratio of phosphatidylserine (PS) and phosphatidylcholine (PC) in presence of Ca2+ was measured by surface plasmon resonance (SPR) analysis. Annexin V-FITC is used for quantification to confirm efficacy of anticancer agent. Induction of apoptosis is induced by
stausrosporine (SSP) as one of anticancer agents. A variety of concentration of SSP induced to be apoptotic cells with macrophage cell line (RAW 264.7 cell) and breast cancer cell line (MCF-7 cell). The apoptotic, necrotic and live cells was monitored using fluorescent probes such as annexin V-FITC and propidium iodide (PI) by confocal microscopy. The fluorescent imaging data
based on confocal microscopy was analysed with intensity of fluorescence for quantification of apoptotic cells.

저자

• Seung Hun Cho [ Dept. of Bionanotechnology ]
• Do Young Cho [ Dept. of Chemical Engineering, Hanyang University ]
• Sang Youn Hwang [ Dept. of Chemical Engineering, Hanyang University ]
• Eun Kyu Lee [ Dept. of Bionanotechnology, Dept. of Chemical Engineering, Hanyang University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576