Earticle

초록

영어

The hair follicle is small, but complex and a dynamic organ. In humans, it plays an important role from the esthetic aspect of social life. When alopecia caused by inheritance, male hormones or stress progresses, the dermal papilla undergoes gradual degradation, resulting in the degeneration of hair follicles. In the past, alopecia was treated typically by various methods of implanting artificial hair into hair follicle root bulbs of the scalp, but such artificial hair implant methods had led to some serious problems, and such methods are now banned. Currently,
there are two methods employed to treat alopecia: drug or natural substance therapy, and human hair transplantation. The drug or natural substance therapy may retard the progress of alopecia or prevent future hair loss, but it may accelerate hair loss when the medication is stopped after a prolonged use. On the other hand, the transplantation of human hair involves taking plugs of natural hair from occipital hair growing areas and transplanting them to bald
areas. Although the transplanted hair settles at the transplant area as a complete hair follicle and becomes a permanent hair that undergoes a normal growth cycle, the number of hair to be transplanted is severely limited, and in case of transplanting about 2,000 hair strands per one operation, it is generally not possible to perform more than three such operations. Thus, the methods currently used for treating alopecia have a number of limitations, and to
overcome such problems, many researchers have attempted to revive hair follicles by in vitro culturing hair follicle cells and implanting them in the treatment area. In our study, by utilizing culture-expanded mesenchymal stem cells (MSCs) which don't have aggregative activity, cell-aggregated spheroidal DP tissues were produced by a special culture condition in vitro, and hair follicle inductive capacity pertinent to the aggregative activity was evaluated. The MSCs cultured as monolayer migrated and formed clumps, the reconstructed DP tissues suspended in the culture medium, their size and shape were similar to intact DP, and the spheroid type composed of similar ECM components could be generated. We were confirmed by light microscope
that the reconstructed DP tissues generated by such procedure had the size and shape similar to actual DP. In addition, by immunohistochemical staining, the expression of laminin and type collagen was also Ⅳ observed, and thus it was confirmed that their ECM components were also similar. In addition, the expression of versican that appears only in cells that could induce hair follicles was confirmed, and by the in vitro mixed culture with hair follicles removed the
hair bulb, it was confirmed that the reconstructed DP-like tissue could form new hair bulb structure by inducing the growth of outer root sheath cells.

저자

• Bo-Young YOO [ Dept. Biochemical and Chemical Engineering, Dongguk University ]
• Yeon-Ho SHIN [ Biomedical Research Center, Lifecord. Co. ]
• Hee-Hoon YOON [ Dept. Biochemical and Chemical Engineering, Dongguk University ]
• Kye-Yong SONG [ Dept. Pathology, College of Medicine, Chung-ang University ]
• Young-Jin Kim [ Biomedical Research Center, Lifecord. Co. ]
• Jung-Keug PARK [ Dept. Biochemical and Chemical Engineering, Dongguk University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576