Earticle

초록

영어

To accomplish the large enhancement in cell number required for the repair of articular cartilage defects, serum is normally supplemented into the expansion medium. However, use of serum has disadvantages such as the risk of disease, lot to lot variations, and the fact that it is difficult to determine what types of substances make up serum makes it unpreferable for use in culture. A
chemically-defined medium will alleviate such dilemmas. Maintenance of the redifferentiation potential of the cells is also important to take into account since chondrocytes tend to lose their phenotype when expanded, thereby producing less glycosaminoglycans and changing their collagen synthesis.1) Following a literature search on serum-free media for chondrocytes, information on growth factors and other components required for supplementation was organized. Using these various supplements, a basal serum-free medium is under development
which will later undergo screening and optimization via statistical methods such as the Plackett Burman approach. Effects on growth and chondrogenic potential will be observed for chondrocytes cultured in the serum-free medium, commercial chondrocyte medium and serum-containing medium.

저자

• Jane Koo [ Dept. of Biological Sciences, KAIST ]
• Byoung-Hyun Min [ Dept. of Orthopedic Surgery, School of Medicine, Ajou University ]
• Gyun Min Lee [ Dept. of Biological Sciences, KAIST ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576