Angelica Gigas extracts were prepared by water boiling, supercritical fluid, and ethanol extraction. Supercritical fluid extract was performed at pressure of 200-400 bar and at temperature of 40-70℃. Inhibitory effect and mechanism of skin aging by Angelica Gigas extracts were investigated using proteome analysis. The human fibroblast were cultured in Dulbecco's Modified Eagle's Medium(DMEM) with or without extracts. The proteome of cultured human fibroblast were profiled using 2D-PAGE. Isoelectric focusing was carried out using commercial immobiline dry strips 3-10L 13cm. SDS-PAGE was performed using 18x24cm, 11% acrylamide gel1). Gels were stained with silver nitrate, analyzed by image master 2D software program. In conclusion, we could identify that many factors such as collagen, integrin beta-1, which affect the skin aging, were regulated by Angelica Gigas extracts.
저자
Mi Ae Yoo [ Department of Applied Biotechnology Ajou University ]
Sang Yo Byun [ Department of Applied Biotechnology Ajou University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동