Earticle

초록

영어

Pipefish, Syngnathus schlegeli (PF) a marine teleost fish, is well known not only for its special medicinal composition and used as one of the most famous and expensive materials of traditional Chinese medicine. It was extracted with water (PFW), methanol (PFM) and ethanol (PFE), respectively and evaluated by various antioxidant assays. The including total antioxidant, DPPH radical scavenging, hydroxyl radical scavenging, superoxide anion radical scavenging, and alkyl radical scavenging. Further, the ROS level was detected using a fluorescence probe,
2’,7’-dichlorofluorescin diacetate (DCFH-DA), which could be converted to highly fluorescent dichlorofluorescein (DCF) with the presence of intracellular ROS on human fibrosarcoma cell (RAW264.7). Those various antioxidant activities were compared tostandard eantioxidants such as α -tocopherol. Among PFM exhibited the highest antioxidant activity in linoleic acid system, effective reducing power, DPPH radical scavenging, hydroxyl radical scavenging, superoxide radical scavenging, alkyl radical scavenging, inhibitory intracellular ROS. Furthermore, MTT assay showed no cytotoxicity on human embryonic lung fibroblasts cell line (MRC-5) and
Murine Leukemia Macrophage cell line (RAW264.7). This antioxidant property depends on concentration and increasing with increased amount of sample.

키워드

저자

• Zhong-Ji Qian [ Department of Chemistry, Pukyong National University ]
• Bo-Mi Ryu [ Department of Chemistry, Pukyong National University ]
• Se-Kwon Kim [ Department of Chemistry, Pukyong National University, Marine Bioprocess Research Center, Pukyong National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576