Earticle

초록

영어

Herein, we propose a novel methodology for efficient recovery of cell-free synthesized recombinant proteins. Through the expression of His-tagged proteins in the presence of Ni-NTA resin, the nascent polypeptides were instantly immobilized on the solid surface, thereby avoiding the inter-molecular aggregation. Upon the elution with imidazole, compared to the reactions
conducted without the resin, it was found that substantially higher amounts of soluble proteins were recovered most likely through improved folding the nascent polypeptides on the surface of resin. We expect that the proposed method will significantly contribute to rapid generation of properly folded proteins for numerous applications.

키워드

저자

• Chang-Gil Park [ Department of Fine Chemical Engineering and Chemistry, College of Engineering, Chungnam National University ]
• Kyung-Ho Lee [ Department of Fine Chemical Engineering and Chemistry, College of Engineering, Chungnam National University ]
• Yong-Chan Kwon [ Department of Fine Chemical Engineering and Chemistry, College of Engineering, Chungnam National University ]
• Ho-Cheol Kim [ Department of Fine Chemical Engineering and Chemistry, College of Engineering, Chungnam National University ]
• Ju-Young Byun [ Department of Fine Chemical Engineering and Chemistry, College of Engineering, Chungnam National University ]
• Kyeong-Ohn Kim [ Department of Fine Chemical Engineering and Chemistry, College of Engineering, Chungnam National University ]
• Jin-Ho Ahn [ School of Chemical and Biological Engineering, College of Engineering, Seoul National University ]
• Dong-Myung Kim [ Department of Fine Chemical Engineering and Chemistry, College of Engineering, Chungnam National University ]

참고문헌

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간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576