Melissa officinalis is perennial herb occurring throughout the east Asia, which have been used for various traditional medicinal treatment. This plant is known to mainly possess rosmarinic acid and protocatechuic acid as polyphnolic acids. To investigate the biological activity of the flavonoids in these plants, it is necessary to develop an effective system for extracting and purifying the flavonoids with possible biological functions. Therefore, in this study, the extraction process with operating variable such as temperature, solvent type and concentration, and solid to solvent ratio was optimized by response surface analysis methodology. Also, the chromatographic purification of rosmarinic acid in the extract obtained from the solvent extraction was carried out by using adsorption chromatography resins such as XADs and Sephadex LH-20 at a variety of conditions. In conclusion, this work is used as basic information for developing an effective purification strategy of rosmarinic acid from plant resources.
저자
Sooah Kim [ Division of Food Bioscience and Technology College of Life Sciences and Biotechnology Korea University ]
Ja Kyong Ko [ Division of Food Bioscience and Technology College of Life Sciences and Biotechnology Korea University ]
Kyoung Heon Kim [ Division of Food Bioscience and Technology College of Life Sciences and Biotechnology Korea University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동