Earticle

초록

영어

The collagen peptides can be extracted from skin, sinew, cartilage, muscles, and the internal organs of cows or pigs1). According to molecular structure and physical chemistry characteristics, the collagen peptides have been used in various industries such as quality foods, medicines and cosmetics2). In the case of fish, collagen peptides are the main component of bone, fin and skin. In this study, we isolated the bioactive peptides related to antioxidative activity and ACE inhibitor activity. The major amino acids found in skate
skin were glycine, glutamic acid and serine, and others contained aspartic acid, arginine and leucine. The major amino acids were contained in more than 50% of the total amino acids. The skate skin hydrolysates were obtained by enzymatic hydrolysis using Alcalase, -chymotrypsin, α Neutrase, pepsin, papain, and trypsin. Among the six hydrolysates, the papain hydrolysate had
the highest DPPH radical scavenging activity compared to other hydrolysates of the skate skin. In addition, the ACE inhibitor activity of the hydrolysates, obtained from the skate skin, using α-chymotrypsin, trypsin, Alcalase, and papain were 75.72%, 65.79%, 63.99%, and 57.35%, respectively. In future studies the peptides with strong antioxidative and ACE inhibitory activity will
be purified from the hydrolysate using consecutive chromatographic methods including sephadex G-25 gel-chromatography and high-performance liquid chromatography. In addition, the purified peptides will be analyzed to identify the amino acid sequences using an amino acid sequencer.

저자

• Heung-Je Kim [ Faculty of Marine Bioscience and Technology, Kangnung National University ]
• Nac-Bum Choi [ Faculty of Marine Bioscience and Technology, Kangnung National University ]
• Joong-Kyung Jeon [ Faculty of Marine Bioscience and Technology, Kangnung National University ]
• Hee-Guk Byun [ Faculty of Marine Bioscience and Technology, Kangnung National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576