Sook-Hee Lee, Eun-Gyeong Lee, Hee-Kyoung Ryu, Chong-Long Wang, Hee-Jung Jang, Pil Kim, Deok-Kun Oh, Seon-Won Kim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A100022
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원문정보
초록
영어
D-Psicose and D-allose, a rare keto and aldo-hexose, are not abundant in nature and are difficult to prepare by chemical methods. For the production of D-allose in E. coli, we used galactose 6-phosphate isomerase coding lacAB of Staphylococcus aureus, Lactococcus lactis, and ribose 5-phosphate isomerase coding rpiB of Clostridium thermocellum, Thermotoga maritima, Corynebacterium glutamicum. Through the whole cell reaction using recombinant E. coli harboring lacAB or rpiB, psicose was not converted to allose, but conversion of allose to psicose was observed. The enzymes used here were shown to have the biased isomerization activity from allose to psicose. In the enzyme reaction using ribose 5-phosphate isomerase of C. thermocellum, which had the highest isomerization activity among the tested enzymes, isomerization of allose to psicose was only occurred.
저자
Sook-Hee Lee [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Eun-Gyeong Lee [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Hee-Kyoung Ryu [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Chong-Long Wang [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Hee-Jung Jang [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Pil Kim [ College of Humanities and Sciences, The Catholic University ]
Deok-Kun Oh [ Department of Bioscience and Biotechnology, Konkuk University ]
Seon-Won Kim [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동