β-Carotene synthesis plasmid pT-HB was used for cotransformation with pSdxs and pSSN12Didi which increased IPP and DMAPP synthesis. β-Carotene production significantly increased 1.5-fold with the amplification of the dxs gene through pSdxs and 4-fold with the mevalonate bottom pathway of pSSN12Didi in the presence of 3.3 mM mevalonate. The pT-DHB, constructed by integrating the dxs gene into pT-HB, was used for co-transformation of E. coli harboring pSSN12Did, resulting into β-carotene production of 141 mg/L. Recombinant E. coli harboring pT-DHB and pSSN12Didi was used to maximize β-carotene production by adjusting the available amounts of glycerol, a carbon source, and mevalonate, the precursor of the mevalonate bottom pathway. When recombinant E. coli was given 16.5 mM mevalonate and 2.5% (w/v) glycerol, β-carotene production of 503 mg/L in concentration was obtained at 144 hours. This work was supported by the EB-NCRC (Grant No. R15-2003-012-02001-0), BioGreen 21 Program (Grant No. 2005041034590), Brain Pool program and BK21 program of Korea.
저자
Sook-Hee Lee [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Eun-Gyeong Lee [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Hee-Kyoung Ryu [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Chong-Long Wang [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Hee-Jung Jang [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Amitabha Das [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
Deok-Kun Oh [ Department of Bioscience and Biotechnology, Konkuk University ]
Seon-Won Kim [ Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동