Gold nanoparticle-mediated immobilization strategy for DNA microarray: a simple and effective method to obtain homogeneous spots with higher capacities over conventional method
Cheulhee Jung, Hyo Young Mun, Taihua Li, Hyun Gyu Park
언어
영어(ENG)
URL
https://www.earticle.net/Article/A100012
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원문정보
초록
영어
We describe here a novel method for the surface immobilization of DNA oligonucleotides using a gold nanoparticle as a mediator. The outer surface of gold nanoparticle is electrostatically attached to amine-modified DNA and, subsequently the gold-DNA complex is easily immobilized on the amine or aldehyde activated glasses through covalent bond or electrostatic force. Based on optimization of gold-DNA concentration and linker effect experiments, sensitivity and specificity of the gold-DNA complex system were tested through hybridization reaction with two kinds of capture probes consisting of different sequences. As a result of the gold-DNA complex system, the surfaces reveal high immobilization efficiency and hybridization intensity for amine-modified DNAs compared with conventional immobilization method. Furthermore, as we use gold nanoparticle as a mediator, it is possible to do silver enhancement enabling us to monitor the capture probe status just after immobilization washing step.
저자
Cheulhee Jung [ Department of Chemical and Biomolecular Engineering, KAIST ]
Hyo Young Mun [ Department of Chemical and Biomolecular Engineering, KAIST ]
Taihua Li [ Department of Chemical and Biomolecular Engineering, KAIST ]
Hyun Gyu Park [ Department of Chemical and Biomolecular Engineering, KAIST ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동