Nae-Rym Lee, Seok Ki Lee, Chang-Soo Lee, BongHyun Chung, Min-Gon Kim
언어
영어(ENG)
URL
https://www.earticle.net/Article/A100005
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원문정보
초록
영어
To detect bacterial pathogens, 16s rRNA was analyzed using a surface plasmon resonance (SPR) biosensor with a signal amplification technology. The 16s rRNA has been used as a genetic marker for identifying organism, and can be analyzed directly without PCR amplification due to relatively high copy number [1]. However, the direct detection of 16s rRNA shows sensitivity limitation compared with PCR-based assays. In this study, a signal enhancing method for high sensitive direct detection of bacterial 16S rRNA using peptide nucleic acid (PNA) probes was developed. The amount of hybridization was monitored by the SPR biosensor, which enables detection of molecular interactions on surface in response to changes in the index of refraction [2]. To amplify the interaction of PNA and 16s rRNA, surface-modified nanoparticles were designed to interact with 16s rRNA selectively. Using this amplification strategy, detection of various pathogens such as E. coli O157, Clostridium perfringens, Staphylococcus aureus was possible.
저자
Nae-Rym Lee [ BioNanotechnology Research Center, KRIBB, Department of Chemical Engineering, Chungnam National University ]
Seok Ki Lee [ BioNanotechnology Research Center, KRIBB ]
Chang-Soo Lee [ Department of Chemical Engineering, Chungnam National University ]
BongHyun Chung [ BioNanotechnology Research Center, KRIBB ]
Min-Gon Kim [ BioNanotechnology Research Center, KRIBB ]
한국생물공학회 [The Korean Society for Biotechnology and Bioengineering]
설립연도
1984
분야
공학>생물공학
소개
이 법인은 생물 공학의 발전과 보급에 이바지하고, 회원 상호 간의 연구 협력과 친목을 도모함을 목적으로 한다
1. 생물공학 분야의 발전을 위한 연구 협력
2. 생물공학의 실용화를 촉진시키기 위한 산학 협동
3. 학술연구 발표회, 강연회, 연수회 등 학술활동의 개최
4. 국,영문 학술지,소식지,학술회의 Proceedings 및 학술도서의 발간
5. 생물공학 발전을 위한 정책 건의
6. 기타 국제 교류 등 본 학회의 목적 달성을 위한 제반 활동