Earticle

초록

영어

Surface Plasmon resonance (SPR)-based sensors have been used to detect the binding etween interaction molecules. In this study, the SPR technology was applied to the cell-based measurement of odorant molecules. Human embryonic kidney (HEK)-293 cells were used as a heterologous cell system. An olfactory receptor protein of rats, I7, was expressed on the surface of HEK-293 cells. For targeting and detecting the protein, rho-tag import sequence was fused with the I7 protein. The interactions between HEK-293 cells expressing I7 and octanal; its specific odorant, elicited the change of Ca2+ ions within the cells and hence the change of Ca2+ ions was detected using SPR. This was confirmed by measuring the cytosolic Ca2+ ions using fluorescent calcium indicator. The SPR response induced by octanal in HEK-293 cells expressing I7 was dependent on the dose of octanal. These SPR responses were regarded as a
result of the intracellular signaling triggered by the binding of odorant molecules to the olfactoryreceptors.

저자

• Sang Hun Lee [ School of Chemical and Biological Engineering, Nano Bioelectronics & Systems Research Center, Seoul National University ]
• Eun Hae Oh [ School of Chemical and Biological Engineering, Nano Bioelectronics & Systems Research Center, Seoul National University ]
• Hyun Seok Song [ School of Chemical and Biological Engineering, Nano Bioelectronics & Systems Research Center, Seoul National University ]
• Tai Hyun Park [ School of Chemical and Biological Engineering, Nano Bioelectronics & Systems Research Center, Seoul National University ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국생물공학회 학술대회

• 간기

  반년간

• 수록기간

  1985~2013

• 십진분류

  KDC 476 DDC 576