Earticle

초록

영어

This study was conducted to investigate the potential use of Cordyceps militaris spent mushroom substrate as a natural antimicrobial agent. The fruiting body and spent mushroom substrate of C. militaris were extracted into 80% ethanol, and the antimicrobial activity was compared. Both extracts (1 mg/disc) exhibited clear antimicrobial activity against Pseudomonas aeruginosa in the agar diffusion assay. The MIC values of the fruiting body and spent substrate extracts against P. aeruginosa were determined to be 0.4 mg/ml and 0.6 mg/ml, respectively, indicating a bacteriostatic action. The inhibitory effects of the C. militarisspent mushroom substrate extract on biofilm formation of P. aeruginosa were determined by biofilm biomass staining and qRT-PCR analysis. The biofilm biomass and cell growth of P. aeruginosa in the cultures treated with 0.2–2.0 mg/ml of extracts were significantly decreasedin a concentration-dependent manner. The qRT-PCR analysis showed that the lasI and lasR gene expression associated to quorum sensing (QS) in the cultures treated with 0.2–2.0 mg/ml of extracts were suppressed in a concentration-dependent manner. Based on the above results, it can be concluded that extracts from C. militaris spent mushroom substrate can be used as an antimicrobial agent derived from natural materials, as demonstrated by the bacteriostatic action and inhibition of biofilm formation of P. aeruginosa.

목차

ABSTRACT
서론
재료 및 방법
사용 균주 및 추출물
P. aeruginosa에 대한 추출물의 항균 활성
P. aeruginosa에 대한 추출물의 최소 성장억제 농도(MIC)와 최소 살균농도(MBC)
P. aeruginosa의 colony morphology 변화
추출물에 의한 P. aeruginosa의 바이오필름 바이오매스(biofilm biomass)형성 억제
추출물에 의한 P. aeruginosa의 quorum sensing 관련 mRNA 발현 억제
통계처리
결과 및 고찰
동충하초 수확후배지 추출물의 항균 활성
P. aeruginosa에 대한 동충하초 수확후배지 추출물의 최소성장 억제 농도 및 최소 살균농도
동충하초 수확후배지 추출물이 P. aeruginosa의 colony morphology에 미치는 영향
동충하초 수확후배지 추출물이 P. aeruginosa의 바이오필름 바이오매스 형성에 미치는 영향
동충하초 수확후배지 추출물이 P. aeruginosa의 QS 관련 mRNA 발현에 미치는 영향
적요
REFERENCES

저자

• 최지현 [ Ji Hyeon Choi | 경상국립대학교 ]
• 조수정 [ Soo Jeong Cho | 경상국립대학교 ] Corresponding Author

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