This study systematically evaluated the anti-inflammatory and whitening activities of Gloeostereum incarnatum, along with metabolite profile changes according to cultivation substrate composition. Crude extract (CE) and solvent-partitioned fractions (HEF, DCMF, EAF) were tested for nitric oxide (NO) inhibition in LPS-stimulated RAW264.7 macrophages, melanin synthesis suppression in α -MSH-induced B16F10 melanoma cells, and cytotoxicity in HCT116 colon cancer cells. Furthermore, primordia grown on various sawdust media supplemented with agricultural by products were analyzed by HPLC to compare metabolite patterns. The DCMF and EAF fractions significantly inhibited NO and melanin production in a concentration dependent manner, without detectable cytotoxic effects at 50 μg/mL. HPLC chromatograms revealed distinct peak profiles depending on substrate composition, with rice bran containing media showing intensified peaks presumed to represent active compounds. These findings indicate that the DCMF and EAF fractions of G. incarnatum hold strong potential as natural agents for inflammation control and skin-whitening, and highlight the importance of substrate formulation in optimizing bioactive metabolite yield.
저자
Ji Min Woo [ Forest Microbiology and Application Division, Department of Forest Bio-resources, National Institute of Forest Science, Suwon, 16631, Korea ]
Yeon Jun Jeong [ Forest Microbiology and Application Division, Department of Forest Bio-resources, National Institute of Forest Science, Suwon, 16631, Korea ]
Kyoung Tae Lee [ Forest Microbiology and Application Division, Department of Forest Bio-resources, National Institute of Forest Science, Suwon, 16631, Korea ]
Aehyeon Nam [ Forest Microbiology and Application Division, Department of Forest Bio-resources, National Institute of Forest Science, Suwon, 16631, Korea ]
Jin Boo Jeong [ Department of Forest Science, Gyeongkuk National University, Andong, Gyeongsangbuk 36729, Republic of Korea ]
Youngki Park [ Forest Microbiology and Application Division, Department of Forest Bio-resources, National Institute of Forest Science, Suwon, 16631, Korea ]
