Earticle

초록

영어

Owing to its diverse range of bioactive compounds, Ganoderma lucidumhas garnered significant research attention for health promotion and disease prevention. Ganodermanondiol, which has a triterpenoid structure, is one of the major active compounds of G. lucidum. In the present study, the anti-inflammatory effects of ganodermanondiol were investigated to evaluate its usefulness as a functional ingredient. Ganodermanondiol (0.5–2 g/mL) significantly inhibited the production of nitric oxide (NO), the expression of the cytokines tumor necrosis factor (TNF)??and interleukin 6 (IL-6), and the expression of cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) in lipopolysaccharide-induced RAW 264.7 (murine macrophage) cells. Ganodermanondiol (0.5–2 g/mL) also inhibited the phosphorylation of mitogen-activated protein kinase (MAPK) signal molecules, including p38 and c-Jun N-terminal protein kinase (JNK) in RAW 264.7 cells. Ganodermanondiol significantly inhibited the essential factors involved in the inflammatory responses of RAW 264.7 cells and would, therefore, serve as a potential prophylactic and therapeutic agent for immune-related diseases.

목차

ABSTRACT
서론
재료 및 방법
시약
세포배양
세포 생존율 평가
RAW 264.7 세포주에 대한 산화질소 (NO) 생성 억제능평가
Total RNA 추출 및 실시간 정량 PCR
단백질 추출 및 웨스턴 블롯
통계 처리
결과 및 고찰
가나도마난디올이 세포 생존율 및 산화질소 (NO) 생성억제에 미치는 영향
가나도마난디올이 염증 매개 인자 발현에 미치는 영향
가나도마난디올이 iNOS 발현 및 MAPK 단백질 인산화에 미치는 영향
적요
감사의 글
REFERENCES

저자

• 박채훤 [ Che-Hwon Park | 건국대학교 의료생명대학 바이오의약학과 ]
• 신주현 [ Ju-Hyeon Shin | 건국대학교 의료생명대학 바이오의약학과 ]
• 박영진 [ Young-Jin Park | 건국대학교 의료생명대학 바이오의약학과 ] Corresponding author

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