Earticle

초록

영어

Cordycepin (3'-deoxyadenosine) is a nucleoside analog known for its diverse range of biological activities. This study investigated the effect of different types of sawdust on the production of the bioactive compound cordycepin. The results of the study showed that different types of wood sawdust affected the biosynthesis of cordycepin and a significant increase was observed when the conventional SDB medium was replaced with 1% NaOH treated pine sawdust. To optimize cordycepin production from Paecilomyces tenuipes in a medium containing 1% NaOH-pretreated pine sawdust, we employed Response Surface Methodology (RSM) in its Box-Behnken design (BBD) canonical form. The optimal conditions were determined as follows: a particle size of 109.5111-mesh (140 m) for 1% NaOH-pretreated pine sawdust, an input weight of 21.1679 g/L, and an incubation time of 73.8423 hours. According to our model, this combination is expected to yield a maximum cordycepin content of 896.1428 g/mL. Experimental validation of this prediction was performed using the suggested optimal conditions, resulting in an average cordycepin content of 922.6771 g/mL across three replicates, thus confirming the model's accuracy.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Preparation of wood sawdust
Effects of the species of plants used for wood sawdust on cordycepin content of P. tenuipes
Pretreatment of wood sawdust
Effect of pretreated wood sawdust for cordycepin content in P. tenuipes on submerged culture
Experimental design using response surface methodology (RSM)
Determination of cordycepin
Statistical Analyses
RESULTS AND DISCUSSION
Effect of Various Sawdust Media on Cordycepin Content
Effect of Pretreatment of Sawdust on Cordycepin Content
Effect of Particle Size and Input Weight of 1% NaOH-pretreated Pine Sawdust on Cordycepin Content
Incubation Time of C. militaris for Cordycepin Production in Medium Containing 1% NaOH-pretreatedPine Sawdust
Optimization of Cordycepin Production in P.tenuipes Cultivation Medium Containing 1% NaOH-pretreatedPine Sawdust Using RSM Based on BBD
ACKNOWLEDGEMENTS
REFERENCES

저자

• Si Young Ha [ Department of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University ]
• Ji Young Jung [ Department of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University ]
• Jae-Kyung Yang [ Department of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University ] Corresponding Author

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