Earticle

초록

영어

Roridin E, a constituent of Podostroma cornu-damae, has excellent medicinal properties and exhibits powerful anticancer effects. However, being a poisonous mushroom, P. cornu-damae is difficult to find in its natural environment. Therefore, only a limited amount of roridin E is isolated from P. cornu-damae. We isolated roridin E from mycelial culture medium of P. cornu-damae and examined its effects on five breast cancer cell lines. The purity of the isolated roridin E was 96%. It exhibited anticancer effects on all five breast cancer cell lines, with an IC50 of at least 0.0006 mg/L. To the best of our knowledge, this is the first report on the investigation of effects of roridin E isolated from the mycelium of P. cornu-damae mushrooms on five breast cancer cell lines. However, safety issues should be investigated and addressed in future studies before roridin E is used in clinical settings.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Microorganism
Submerged culture
Extract and isolation of roridin E
Analytical TLC and UV-Visible spectroscopy
High pressure liquid chromatography analysis
Antioxidant properties
Cell lines and culture
The MTT cell viability assay for cytotoxicity testing
Scratch wound healing assay
RESULTS AND DISCUSSION
Isolation of roridin E from P. cornu-damae culture broth
Characterization of the isolated compound used UVVisiblespectroscopy
HPLC spectrum of isolated roridin E
Antioxidant activity of isolated roridin E
Anticancer activity of isolated roridin E
CONCLUSION
ACKNOWLEDGEMENTS
REFERENCES

저자

• Si Young Ha [ Department of Environmental Materials Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Dong Hwan Lee [ Forest Medicinal Resources Research Center, National Institute of Forest Science, Youngju 36040, Republic of Korea ]
• Ji Young Jung [ Department of Environmental Materials Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Han Min Park [ Department of Environmental Materials Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Jae-Kyung Yang [ Department of Environmental Materials Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ] Corresponding Author

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