Earticle

초록

영어

Volvariella bombycinais an edible mushroom commercially cultivated in Korea and other countries. In this study, the culture characteristics and optimal growth conditions of milky mushroom strains were determined. The growth temperature and pH range of V. bombycinawas extensively investigated between 15 and 35oC and pH 3–11. For efficient cultivation, 20 types of nutrient sources were selected, specifically, 21 carbon sources, 6 organic nitrogen sources, 7 inorganic nitrogen sources, 13 amino acids, 9 organic acids, and 13 inorganic salts. The impact of each of the selected nutrition sources and their concentration on growth was investigated. The optimum temperature and pH were determined to be 30oC and pH 8.0, respectively. The optimum concentration of medium elements required for mycelial growth of V. bombycinawas determined to be as follows: carbon source, 0.7% Soluble starch; organic nitrogen source, 0.7% Soytone; inorganic nitrogen source, 1.0% (NH4)2C4H4O6; amino acid, 0.1% cysteine; organic acid, 0.07% lactic acid; and inorganic salt, 0.07 mM CaCl2.

목차

ABSTRACT
서론
재료 및 방법
시험균주 및 배양배지
Genomic DNA 분리
ITS primer의 PCR
유전적 유연관계 분석 ITS
균사배양적온 선발
균사배양적합 초기 pH 선발
균사배양적합 영양원 선발
통계처리
결과 및 고찰
ITS 염기서열에 의한 유연관계 분석
온도와 pH에 따른 균사배양 특성
영양원에 따른 균사배양 특성
적요
감사의 말씀
REFERENCES

저자

• 민경진 [ Gyeong Jin Min | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 박혜성 [ Hye Sung Park | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 하태문 [ Tai Moon Ha | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 이은지 [ Eun Ji Lee | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ] Corresponding author

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