Earticle

초록

영어

This study aimed to verify the whitening effect of Cordyceps militaris, which is distributed in several countries worldwide, including Korea, Japan, and China, and has various medical effects. To screen the efficacy of C. militaris, the inhibitory activity of tyrosinase, which was 66% at a concentration of 1 mg/mL, was measured. Thereafter, the survival rate of melanoma cells was measured, and cell experiments were conducted at a concentration of 90% or more in which C. militaris was not toxic to cells. After measuring the inhibitory effect of TRP-1, TRP-2, tyrosinase protein, and mRNA expression, which are factors influencing melanin synthesis, C. militaris was found to decrease in all factors, with an expression level that was significantly lower compared to quercetin. This confirmed that C. militaris stimulated with LED has excellent whitening activity and can be used as a functional whitening cosmetics material.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Fungal strains
Submerged culture conditions
Cell culture
Cell viability assay
Tyrosinase activity assay
Melanin content assay
Investigation of genes
Investigation of protein expression
RESULTS AND DISCUSSION
Cell viability of melanoma cells induced by C. militaris mycelia
Inhibition mushroom tyrosinase activity of C. militaris mycelia
Effect of C. militaris mycelia on melanin content in B16F10 cells
Genetic investigation
Investigation of protein expression
CONCLUSION
Acknowledgements
Data Availability
Conflicts of Interest
Authors Contributions
REFERENCES

저자

• Si Young Ha [ Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Ji Young Jung [ Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Jae-Kyung Yang [ Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ] Corresponding Author

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