Earticle

초록

영어

Cordyceps militaris mycelium extracts containing high amounts of cordycepin were evaluated in vitro for their antiinflammatory and tumor cell growth-inhibitory activities. All extracts dose dependently inhibited the increased production of inflammatory mediators including reactive oxygen species (ROS), nitric oxide (NO), and β-hexosaminidase in lipopolysaccharide (LPS)-stimulated inflammatory cells. All extracts were evaluated for anti-proliferative activity against normal RBL-2H3 cells and diverse types of cancer cell lines, including HCT, MC5-7, U-87MG, AGS, and A549 cells. The extract showed the strongest growth inhibition (IC50 = 28.13 μg/mL) relative to vehicle-treated control cells against fibrosarcoma (MC5-7). We have demonstrated anti-inflammatory activity of C. militaris via inhibition of NO, ROS production, and β-hexosaminidase release in activated cells. C. militaris mycelium extract was also evaluated mechanistically and found to exert six types of anti-cancer activity, confirming its pharmacological potential. Our study suggests C. militaris use as a potential source of anti-inflammatory and anticancer agents. C. militaris may also be considered a functional food.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Fungal strains
Submerged culture conditions
Mycelium collection and ultrasonic-assisted extraction
Determination of cordycepin
Cell cultures
Cell viability
The β-hexosaminidase assay
ROS assay
NO assay
Statistical analyses
RESULTS AND DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES

저자

• Si-Young Ha [ Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ]
• Ji-Young Jung [ Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ]
• Jae-Kyung Yang [ Department of Environmental Materials Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ] Corresponding author

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