Earticle

초록

영어

This study was conducted to develop food and medicinal products containing useful components of Lentinula edodes in Codonopsis lanceolata and Platycodon grandiflorus for use as herbal medicine. We manufactured C. lanceolata (FCLM) and P. grandiflorus (FPLM) extract fermented with L. edodes mycelium. The effect of the two fermented products on proximate composition, free sugar, organic acid, β-glucan, ergothioneine, ergosterol, and vitamin D2 levels, and 3T3-L1 preadipocyte cell growth were studied. The proximate composition analysis results showed that the crude fiber and crude fat content in FCLM was higher than that in FPLM, and the crude protein and soluble nitrogen content in FPLM was higher than that in FCLM. Free sugar analysis detected arabinose, glucose, and sucrose in both FCLM and FPLM, and the total free sugar content was high in FPLM. The organic acid content was lower in FCLM and FPLM compared to C. lanceolata and P. grandiflorus before fermentation. The β-glucan content was higher than that of L. edodes used as a control in both fermented products, FCLM and FPLM. The content of ergothioneine, an antioxidant, was higher in FCLM than in FPLM. Ergosterol content was highest in L. edodes which was used as a control, and the two fermented products showed similar content. Vitamin D2 was detected only in FCLM and FPLM, and FPLM (0.58±0.01 mg%) showed a higher vitamin D2 content than FCLM (0.47±0.01). FCLM and FPLM showed a higher level of cell viability for 3T3-L1 pre-adipocytes compared to non-fermented C. lanceolata and P. grandiflorus. In addition, FCLM and FPLM inhibited 3T3-L1 preadipocyte differentiation more than C. lanceolata and P. grandiflorus before fermentation, which may exert an anti-obesity effect.

목차

ABSTRACT
서론
재료 및 방법
재료
시약
표고균사배양물 제조
일반성분 분석
유리당 분석
유기산 분석
β-Glucan 함량 분석
Ergothioneine 함량 분석
Ergosterol 함량 분석
Vitamin 분석
3T3-L1 세포에서 시료의 세포독성 확인
Oil Red O staining을 통한 지방세포 분화 저해능 분석
통계 처리
결과 및 고찰
일반성분
유리당 함량
유기산 분석
β-Glucan 함량
Ergothioneine 함량
Ergosterol 함량
Vitamin D2 함량
지방전구세포 독성
지방전구세포 분화 억제
적요
감사의 글
REFERENCES

저자

• 고영우 [ Young-Woo Koh | (재)장흥군버섯산업연구원 ]
• 윤경원 [ Kyeong-Won Yun | 순천대학교 바이오한약자원학과 ]
• 김경제 [ Kyung-Je Kim | (재)장흥군버섯산업연구원 ]
• 진성우 [ Seong-Woo Jin | (재)장흥군버섯산업연구원 ]
• 임승빈 [ Seung-Bin Im | (재)장흥군버섯산업연구원 ]
• 하늘이 [ Neul-I Ha | (재)장흥군버섯산업연구원 ]
• 정희경 [ Hee-Gyeong Jeong | (재)장흥군버섯산업연구원 ]
• 김승주 [ Seung-Ju Kim | 데이앤바이오(주)농업회사법인 ]
• 김복선 [ Bok-Seon Kim | 데이앤바이오(주)농업회사법인 ]
• 최유진 [ Yu-Jin Choi | (재)임실치즈앤식품연구소 ]
• 송다혜 [ Da-Hye Song | (재)임실치즈앤식품연구소 ]
• 서경순 [ Kyoung-Sun Seo | (재)장흥군버섯산업연구원 ] Corresponding Author

참고문헌