Earticle

초록

영어

The purpose of this study was to identify and characterize the laccase genes of Flammulina velutipes var. lupinicola. Five laccase genes (g1934, g1937, g2415, g2539, g5858) were selected based on the copper binding site and signal peptide analysis results using the laccase gene selected from the F. velutipes var. lupinicola genome. The size of the laccase genes of F. velutipes var. lupinicola were 1,488 bp~1,662 bp. As a result of cDNA sequence analysis, 14 to 17 introns were identified in the laccase genes. The cleavage site predicted as the signal peptide of the laccase gene was found to be located between 20 bp and 34 bp from the N-terminus. In addition, separation and purification were performed to characterize the F. velutipes var. lupinicola laccases, and the optimal activity of the separated and purified proteins were analyzed by pH, temperature and time. Five bands with laccase activity were found from zymogram analysis. The optimal pH of the reaction was 5.5, the optimal temperature was found to be 40oC. Therefore, characterization of the laccase genes identified in this study should help in better understanding the biomass decomposition of F. velutipes var. lupinicola.

목차

ABSTRACT
서론
재료 및 방법
시험균주 및 배양
생물정보학 기반 laccase 유전자 분석
RNA 추출, cDNA 합성 및 염기서열 분석
Laccase 단백질 농축 및 이온 교환 크로마토그래피, 소수성 상호작용 크로마토그래피에 의한 분리
단백질 정량 및 laccase 효소 활성 평가
SDS-PAGE 및 zymogram 분석
온도, pH, 시간별 Laccase 효소 활성 분석
결과 및 고찰
Flammulina velutipes var. lupinicola의 유전체 정보 기반 신규 laccase 유전자 선발 및 유전적 특성 규명
Flammulina velutipes var. lupinicola의 laccase 효소활성 평가
적요
감사의 글
REFERENCES

저자

• 유혜원 [ Hye-Won Yu | 건국대학교 글로컬캠퍼스 의료생명대학 바이오의약학과 ]
• 박영진 [ Young-Jin Park | 건국대학교 글로컬캠퍼스 의료생명대학 바이오의약학과 ] Corresponding Author

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