Earticle

초록

영어

Currently, button mushroom, Agaricus bisporus is one of the most consumed mushroom in the world. However, despite of its importance in food market, molecular genetic modification method for breeding of A. bisporus is not well established. In this study, we optimized yield of A. bisporus protoplast with Lysing enzyme, Chimax-N and cellulase. With this composition, 1.0 × 108/mL of protoplasts were obtained reliably. PEG-mediated transformation with spermidine showed almost 100-fold higher yield than non-spermidine method.

목차

ABSTRACT
적 요
REFERENCES

저자

• 김민식 [ Minseek Kim | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 장갑열 [ Kab-yeul Jang | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 이윤상 [ Yun-Sang Lee | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 오민지 [ Min Ji Oh | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 임지훈 [ Ji-Hoon Im | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 오연이 [ Youn-Lee Oh | 농촌진흥청 국립원예특작과학원 버섯과 ] Corresponding Author

참고문헌