Earticle

초록

영어

In this study, we evaluated the anti-inflammatory effect of extract from Cudrania tricuspidata twig sawdust fermented with Ganoderma lucidum mycelium. Fermented Cudrania tricuspidata twig sawdust extracted with 70% ethanol and elucidated the potential signaling pathway in lipopolysaccharide (LPS)-induced RAW264.7 cells. Fermented Cudrania tricuspidata twig sawdust inhibits LPS-stimulated nitric oxide (NO) production without affecting cell viability in a dose-dependent manner and production of LPS-induced pro-inflammatory cytokines such as interleukin (IL)-1β, tumor necrosis factor (TNF)-α and prostaglandin2 (PGE2). Fermented Cudrania tricuspidata twig sawdust also suppressed the expression of the pro-inflammatory mediators such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW264.7 cells. Moreover, Fermented Cudrania tricuspidata twig sawdust significantly attenuated LPS-induced IkappaB (IκB) degradation and suppressed nuclear factor kappa B (NF-κB) nuclear translocation. These results suggest that fermented Cudrania tricuspidata twig sawdust may have great potential for the development of anti-inflammatory agent.

목차

ABSTRACT
서 론
재료 및 방법
시료 및 균주
꾸지뽕나무 잔가지 발효 및 추출물 제조
세포배양
세포 독성 측정
Nitric oxide (NO) 생성량 측정
Prostaglandin E2 (PGE2) 및 inflammatory cytokine생성량 측정
Western blot 분석
Cytoplasmic and nuclear fraction
통계처리
결과 및 고찰
Raw264.7 세포에 대한 세포독성
NO 및 PGE2 생성에 미치는 영향
inflammatory cytokine 생성에 미치는 영향
iNOS 및 COX-2 단백질 발현에 미치는 영향
NF-κB 활성화 조절에 미치는 영향
적 요
REFERENCES

저자

• 박세은 [ Se-Eun Park | 광주대학교 식품생명공학과 ]
• 김명곤 [ Myung Kon Kim | 전북대학교 식품공학과 ]
• 김승 [ Seung Kim | 광주대학교 식품생명공학과 ] Corresponding Author

참고문헌