Earticle

초록

영어

This study was conducted to improve the useful components and biological activity of Lentinula edodes fermented by lactic acid bacteria (LAB). Three LAB strains (Lactobacillus brevis KCCM 11904, L. plantarum KCCM 354469, and L. fermentum KCCM 12116) were inoculated and used for L. edodes hot water extract (10%, 20%, 30%) fermentation. LAB fermentation of L. edodes hot water extracts decreased pH and thus were more acidic than non-fermented L. edodes hot water extract. β-glucan and ergothioneine contents were increased by L. edodes in a concentration-dependent manner. The ergothioneine and β-glucan contents were highest in fermented with 30% L. edodes hot water extract fermented by L. plantarum and L. brevis (40.48 mg/ 100 g and 13.94%, respectively). The hepato-protective effect of fermented L. edodes hot water extracts by the three LAB were tested using Sprague-Dawley rat primary hepatocytes. In primary hepatocytes obtained following liver injury induced by acetaminophen, fermented L. edodes hot water extracts by the three LAB showed protective effects, as evident by reduction of the aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase liver markers. The collective results indicate that the fermented L. edodes hot water extracts obtained using LAB are potentially valuable in preventing or treating liver disease.

목차

ABSTRACT
서 론
재료 및 방법
재 료
시약
표고 유산균 발효물의 제조
pH 및 적정산도 측정
Ergothioneine 함량 분석
β-Glucan 함량 분석
실험동물
일차 간세포 배양(Primary hepatocyte culture)
일차 간세포에서 시료의 세포독성 확인
생화학적 분석
통계분석
결과 및 고찰
표고추출농축액의 유산균 발효에 의한 특성 변화
표고 유산균 발효물의 유용성분 함량
표고 유산균 발효물의 일차 간세포에서의 독성
표고 유산균 발효물의 간세포 보호 효과
적 요
REFERENCES

저자

• 임승빈 [ Seung-Bin Im | 장흥군버섯산업연구원 ]
• 김경제 [ Kyung-Je Kim | 장흥군버섯산업연구원 ]
• 진성우 [ Seong-Woo Jin | 장흥군버섯산업연구원 ]
• 고영우 [ Young-Woo Koh | 장흥군버섯산업연구원 ]
• 하늘이 [ Neul-I Ha | 장흥군버섯산업연구원 ]
• 정희경 [ Hee-Gyeong Jeong | 장흥군버섯산업연구원 ]
• 이재근 [ Jae-Keun Lee | 장흥군버섯산업연구원 ]
• 윤경원 [ Kyeong-Won Yun | 순천대학교 바이오한약자원학과 ]
• 서경순 [ Kyoung-Sun Seo | 장흥군버섯산업연구원 ] Corresponding Author

참고문헌