Earticle

초록

영어

In this study, monokaryons of “Heukari” (Pleurotus ostreatus) and “Hosan” (Pleurotus pulmonarius) were separated to remove the cell wall, and a cross-species protoplast fusion was developed through chemical treatment with polyethylene glycol . The protoplast-fused PF160306 and PF160313 strains have a culture period of 10 and 2 days shorter than that of the “Heuktari” and “Hosan” cultivars, respectively. Furthermore, the growth of the strains was faster than that of the existing cultivars. The yield was 135.9 g per bottle, which was approximately 8% higher than that of the commercially available “Hosan” cultivar; however, it was not statistically significant. A growth survey was conducted after treatment at five temperatures (15, 18, 21, 23, and 25oC). The growth of the strains accelerated with the increase in temperature. However, at 21oC, the yellow color of pileus was the brightest. Band pattern, assessed using URP Primer 7, was similar to that of the “Hosan” cultivar. The DPPH radical scavenging capacity and polyphenol content were 62.5% and 43.5 mg/mL, respectively, for “Sunjung” and 65.7% and 49.9 mg/mL, respectively, for PF160313. Furthermore, the antihypertensive activities of the “Sunjung” cultivar and PF160313 were similarly high at 74% and 75%, respectively. In conclusion, cross-species hybridization via the protoplast fusion technique can be used for obtaining primary data for mushroom breeding to develop new varieties. In addition, the protoplast fusion technique might aid in expanding the market for yellow mushrooms.

목차

ABSTRACT
서 론
재료 및 방법
실험 재료
원형질체 융합
생육용 배지 제조 및 자실체 발생
원형질체 융합계통의 재배적 특성 조사
자실체 특성 조사
항산화활성 분석
항고혈압활성(ACE; Angiotensin I-converting enzyme) 분석
베타글루칸 분석
URP-PCR 분석
통계처리
결과 및 고찰
원형질체 융합계통 육성과 자실체 형태
원형질체 융합계통 균사 특성
원형질체 융합계통 재배 및 자실체 형태적 특성
생육온도에 따른 원형질체 융합계통 재배기간 및 자실체 특성 변화
융합 확인을 위한 원형질체 융합계통 URP-PCR 분석과대선 형성
항산화 활성 분석
항고혈압활성 측정
베타글루칸 분석
적 요
REFERENCES

저자

• 권희민 [ Hee-Min Gwon | 국립농업과학원 농식품자원부 발효가공식품과 ]
• 이윤혜 [ Yun-Hae Lee | 국립농업과학원 농업환경부 토양비료과 ]
• 김정한 [ Jeong-Han Kim | 경기도농업기술원 친환경미생물연구소 ]
• 백일선 [ Il-Sun Baek | 경기도농업기술원 친환경미생물연구소 ]
• 강희완 [ Hee-Wan Kang | 한경대학교 생명공학부 원예생명공학전공 ]
• 최종인 [ Jong-In Choi | 경기도농업기술원 환경농업연구과 ] Corresponding Author

참고문헌