Earticle

초록

영어

In order to determine the changes in microbial community due to termites, soil microorganisms surrounding the termites were investigated. First, bacterial communities from soil with termites collected at Seonamsa temple, Suncheon city, Korea were compared by next-generation sequencing (NGS, Illumina Miseq). The bacterial composition of soil from Daeungjeon without termites and the soil from Josadang, Palsangjeon, and Samjeon with termites were compared. Next, the bacterial composition of these soils was also compared with that of humus soil cultured with termites. A total high-quality sequences of 71,942 and 72,429 reads were identified in Seonamsa temple’s soil and humus soil, respectively. The dominant phyla in the collected Seonamsa temple’s soil were Proteobacteria (27%), Firmicutes (24%) and Actinobacteria (21%), whereas those in the humus soil were Bacteriodetes (56%) and Proteobacteria (37%). Using a two-dimensional plot to explain the principal coordinate analysis of operational taxonomic unit compositions of the soil samples, it was confirmed that the samples were divided into soil with and without termites, and it was especially confirmed that the Proteobacteria phylum was increased in humus soil with termites than in humus soil without termites.

목차

ABSTRACT
1. INTRODUCTION
2. MATERIALS AND METHODS
2.1. Soil sampling location and humus soil test for NGS analysis
2.2. Next Generation Sequencing (NGS) analysis
3. RESULTS
3.1. Estimated richness and diversity of soil microbiota based on NGS analysis
3.2. Structural composition of soil microbiota at the phylum level
3.3. Correlation relationship of samples using multidimensional analysis
4. DISCUSSION
ACKNOWLEDGEMENTS
REFERENCES

저자

• Young Hee Kim [ Restoration Technology Division, National Research Institute of Cultural Heritage, Daejeon 34122, Korea ] Corresponding Author
• Boa Lim [ Restoration Technology Division, National Research Institute of Cultural Heritage, Daejeon 34122, Korea ]
• Jeung Min Lee [ Restoration Technology Division, National Research Institute of Cultural Heritage, Daejeon 34122, Korea ]
• Jin Young Hong [ Restoration Technology Division, National Research Institute of Cultural Heritage, Daejeon 34122, Korea ]
• Soo Ji Kim [ Restoration Technology Division, National Research Institute of Cultural Heritage, Daejeon 34122, Korea ]
• Ji Hee Park [ Restoration Technology Division, National Research Institute of Cultural Heritage, Daejeon 34122, Korea ]

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