Earticle

초록

영어

Roridin E, produced by Podostroma cornu-damae, is a mycotoxin with anticancer activity. To increase the content of roridin E, submerged culture conditions were optimized using response surface methodology. Three factors, namely, medium initial pH, incubation time and agitation speed were optimized using a Box–Behnken design. The optimum submerged culture conditions to increase the content of roridin E included a medium with an initial pH of 4.0, an incubation time of 12.90 days, and an agitation speed of 63.03 rpm. The roridin E content in the submerged culture, under the aforementioned conditions, was 40.26 mg/L. The findings of this study can help lower the current price of roridin E and promote its related research.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Reagents and standards
Microorganism
Culture media
Submerged Culture
Determination of mycelial dry weight
Determination of roridin E
Experimental design by the RSM
Statistical analysis
RESULTS AND DISCUSSION
Effect of various media on mycelial dry weight and roridin E production
One-factor-at-a-time experiment Effect of incubation time on roridin E production
Effect of initial pH on roridin E production
Effect of agitation speed on roridin E production
Optimization of roridin E production by the RSM
ACKNOWLEDGEMENTS
REFERENCES

저자

• Dong Hwan Lee [ Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Si Young Ha [ Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Ji Young Jung [ Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]
• Jae-Kyung Yang [ Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ] Corresponding Author

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