Earticle

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영어

To develop a method for the differentiation of Pleurotus ostratus cultivars, the multiplex-simple sequence repeat (SSR) primer set based on the SSRs obtained from whole genomic DNA sequence analysis was designed with two polymerase chain reaction (PCR) primer sets. These SSR primer sets were employed to distinguish 10 cultivars and strains. Twenty polymorphic markers were selected based on the genotyping results. PCR with each primer produced 1–4 distinct bands ranging in size from 150 to 350 bp, which was within the expected range. However, since a sole SSR marker was unable to detect polymorphisms in every cultivar, multiplex PCRs with composite PCR primer sets were employed. The multiplex primer, “166+115,” completely discriminated 12 cultivars and strains with 40 loci, which were 12 more than the simple arithmetic addition of each locus of the primers 115 and 166. These results might be useful to provide an efficient method for the differentiation of P. ostreatus cultivars with separate PCRs for the quality control of spawn and protection of breeders’ rights.

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저자

• 최종인 [ Jong In Choi | 경기도농업기술원 버섯연구소 ]
• 정화진 [ Hwa Jin Jung | 한국농수산대학 버섯학과 ]
• 나경숙 [ Kyeong sook Na | 한국농수산대학 버섯학과 ]
• 오민지 [ Min-Ji Oh | 농촌진흥청 버섯과 ]
• 김민근 [ Min-Keun Kim | 경남농업기술원 ]
• 류재산 [ Jae-San Ryu | 한국농수산대학 버섯학과 ] Corresponding Author

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