Earticle

초록

영어

The genome sequence of various Flammulina species has recently been reported, thereby revealing a diverse genetic repertoire. In this study, we identified laccase genes and analyzed their structural characteristics in Flammulina elastica (F. elastica) genome. Through genome analysis and bioinformatics approaches, three laccase genes (Fe-lac1, -lac2, and -lac3) were identified, ranging from 1,548 to 1,602 bp in length. These genes contained a copper ion-binding region with ten histidine residues and one cysteine residue and a disulfide bond-forming region with four cysteine residues. Full-length cDNA sequencing analysis revealed that laccase genes contain 12 to 16 introns and signal peptides between 17 and 22 bp in the N-terminus. Structural characterization of the laccase genes identified in this study should help in better understanding the biomass decomposition of F. elastica.

목차

ABSTRACT
서론
재료 및 방법
시험균주 및 배양
생물정보학 기반 laccase 유전자 분석
RNA 추출, 전장 cDNA 합성 및 염기서열 분석
염기서열 분석
결과 및 고찰
F. elastica의 laccase 유전자
적요
감사의 글

저자

• 유혜원 [ Hye-Won Yu | 건국대학교 글로컬캠퍼스 의료생명대학 바이오의약학과 ]
• 박영진 [ Young-Jin Park | 건국대학교 글로컬캠퍼스 의료생명대학 바이오의약학과 ] Corresponding Author

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