Earticle

초록

영어

The fatty acid binding protein 4 (FABP4) gene plays an important role in lipid metabolism and homeostasis in adipocytes. The objective of this study was to analyze the association between a single nucleotide polymorphism (SNP), g.7516G>C, in the FABP4 gene and economic traits of Korean native cattle, Hanwoo. Primers were designed to target a region of the FABP4 gene between nucleotides 7417 and 7868 (AAFC01136716). The SNP, which was detected by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) method using restriction enzyme MspA1I, was genotyped in 319 animals of Hanwoo steer population. Statistical analysis showed that the SNP genotype of the FABP4 gene significantly affected carcass weight (CW, p<0.01), longissimus muscle area (LMA, p<0.001), and marbling score (MS, p<0.001). GG allele of the SNP on 246 animals in a Wagyu × Limousin F2 reference population showed a higher MS (p<0.05) and subcutaneous fat depth (p<0.05) in previous report. But CC allele of the SNP showed greater values for MS, LMA, and CW in Hanwoo steers. These results suggest that the g.7516G>C SNP located in the FABP4 gene may affected differently depending on the cattle breed and can be used as a genetic selection marker in Korean native cattle.

목차

ABSTRACT
Ⅰ. Introduction
Ⅱ. Materials and Methods
1. Animals and data collection
2. DNA extraction
3. PCR-RFLP genotypes
4. Statistical analysis
Ⅲ. Results and discussion
Ⅳ. References

저자

• 이승규 [ Seung-Kyoo Lee | 강원대학교 동물생명과학대학 대학원생 ]
• 이영섭 [ Young-Sub Lee | 강원대학교 동물생명과학대학 대학원생 ]
• 김훈 [ Hun Kim | 강원대학교 동물생명과학대학 대학원생 ]
• 배윤호 [ Yun-Ho Bae | 강원대학교 동물생명과학대학 대학원생 ]
• 이성진 [ Sung-Jin Lee | 강원대학교 동물생명과학대학 교수 ] Corresponding author

참고문헌