Earticle

초록

영어

Termitomyces albuminosus has been recognized to have the best mushrooms in China, in terms of taste and aroma. The efficacy of these mushrooms has been recorded in the botanical list. However, research on the development of their artificial culture methods is necessary. In this study, we prepared an organic solvent extract and a hot water extract to understand the development of compounds and functional foods with antioxidant and anti-inflammatory activities. The IC50 value of DPPH radical scavenging activity of the hot water extract (TA4) was 1.5 mg/mL and the IC50 value of the MeOH fraction (TA2) was 1.93 mg/mL. The anti-inflammatory activity was investigated by the inhibition of NO production. EtOAc fraction (TA1) is a crude extract, but 79% of NO production was inhibited at 100 μg/mL. NO was not produced at 200 μg/mL. TA1-5-6, from TA1 inhibited NO production by 15% as compared to the positive control at 15 μg/mL, and completely inhibited NO production at 30 μg/mL. No cytotoxicity was observed at 50 μg/mL. TA2-1-5 from the MeOH fraction (TA2) inhibited more than 75% of NO production at 30 μg/mL; cytotoxicity was very low even at 50 μg/mL. In conclusion, by selective solvent selection, it was possible to manufacture an extract with no cytotoxicity and excellent biological activities. Furthermore, the extracts showed potential for developing various functional foods and drugs.

목차

ABSTRACT
서론
재료 및 방법
실험 재료
계종버섯의 용매 추출과 열수 추출
계종버섯 분획물에서 크로마토그래피법을 이용한 물질분리
항염증 활성 확인을 위한 세포 배양
계종버섯 추출물 및 분획물의 세포에 대한 독성 확인
계종버섯 추출물 및 분획물의 nitric oxide (NO) 생성억제 활성 확인
계종버섯 추출물과 분획물의 DPPH 라디칼 소거능 측정
계종버섯 추출물과 분획물의 ABTS 라디칼 소거능 측정
통계처리
결과 및 고찰
용매별 추출물의 수율
계종버섯 추출물과 분획물의 항산화 활성 측정
계종버섯 추출물과 분획물의 세포 독성과 항염증 활성측정
TA1으로부터 항염증 물질 분리
TA2로부터 항염증 물질 분리
적요
감사의 글
REFERENCES

저자

• 강민재 [ Min-Jae Kang | 부경대학교 미생물학과 ]
• 김영재 [ Young-Jae Kim | 부산대학교 생명환경화학과 ]
• 황현정 [ Hyeon-Jeong Hwang | 부산대학교 생명환경화학과 ]
• 김문호 [ Moon-Ho Kim | 부산대학교 생명환경화학과 ]
• 신종무 [ Jong-Moo Sin | 농업회사법인 ㈜힘찬 ]
• 신평균 [ Pyung-Gyun Shin | 농업회사법인 ㈜힘찬 ]
• 김군도 [ Gun-Do Kim | 부경대학교 미생물학과 ] Corresponding Author
• 김근기 [ Keun Ki Kim | 부산대학교 생명환경화학과 ] Corresponding Author

참고문헌