Earticle

초록

영어

Light is an important factor for cordycepin production in Cordyceps militaris. We investigated the effects of different light-emitting diode (LED) conditions including various LED wavelengths and their combinations on cordycepin production in Cordyceps militaris cultivated in submerged culture. The results of our study showed that the combinations of LED wavelengths were more beneficial than single LED sources for cordycepin production. Among the three tested wavelength combinations, the greatest effects for cordycepin production were observed for the red:blue light combination at the wavelength ratio of 5:5 or 3:7. The optimal culture conditions were 19.2278 h/day of illumination time; 9.19497 g/50 mL of glucose content in the media; and 53.112 h of cultivation time. Our model predicted a maximum yield of 2860.01 μg/mL cordycepin. Finally, to verify the calculated maximum, we performed experiments in the culture media representing the obtained optimum combination and the cordycepin yield of 2412.5 μg/mL.

목차

ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Reagents and standards
Mycelia preparation
Submerged culture
Different wavelengths of LEDs
Combination of different ratios of wavelength of LEDs
Illumination time
Glucose content in media
Determination of mycelia dry weight
Determination of cordycepin
Experimental design by RSM
Statistical analysis
RESULTS AND DISCUSSION
Effect of various media on mycelia dry weight and production of cordycepin 
Effect of LEDs on mycelia dry weight and cordycepin content
Effects of LEDs combination on the mycelia dry weight and cordycepin content
Optimization of cordycepin production by RSM(BBD)
ACKNOWLEDGEMENTS
REFERENCES

저자

• Si-Young Ha [ Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ]
• Ji-Young Jung [ Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ]
• Jai-Hyun Park [ Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ]
• Dong-Hwan Lee [ Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ]
• Ji-Won Choi [ Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ]
• Jae-Kyung Yang [ Division of Environmental Forest Science/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Republic of Korea ] Corresponding Author

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