Earticle

초록

영어

Sparassis latifolia is a fungus abundant in β-glucan and amino acids and is highly valued as a medicinal mushroom. Among amino acids, γ-aminobutyric acid (GABA) is a free amino acid and has biological effects, such as increase/decrease of hypertension, improvement of cerebral blood flow, and prevention of dementia. In this study, biological elicitors were used to increase bioactive substances as a biofortification method. Sodium alginate extracted from seaweed (Sargassum horneri, Sargassum fulvellum, Sargassum fusiforme) were used as the elicitor. The levels of β-glucan and GABA in the mycelium and fruiting body grown by adding the elicitor to the medium were investigated. Addition of sodium alginate positively affected GABA production and negatively affected the β-glucan production in these fungi. Sodium alginates extracted from S. fulvellum induced the highest increase in GABA in the mycelium and fruiting bodies. Moreover, we investigated the effects of the extracts from mycelium and fruiting bodies on dendrite development in primary cortical neurons. We found that the extract from the fruiting bodies of sodium alginate treated fungi with increased levels of GABA inhibited the dendrite outgrowth of excitatory neurons, but not inhibitory neurons.

목차

ABSTRACT
서 론
재료 및 방법
꽃송이버섯 공시균주 및 생장
해조류 기반 엘리시터 제조
β-Glucan 측정
GABA 측정
초대 신경세포 배양
면역형광법
MTT assay
Western blotting
Dendrite outgrowth 측정
통계
결과 및 고찰
β-Glucan 측정결과
GABA 측정결과
독성실험결과
초대 신경세포 수상돌기 발달 결과
신경세포 내 신호전달경로의 변화결과
결 론
REFERENCES

저자

• 최문희 [ Moon-Hee Choi | 조선대학교 공학기술연구원 ]
• 기성환 [ SungHwan Ki | 조선대학교 약학대학 ]
• 이성은 [ Seong-Eun Lee | 조선대학교 약학대학 ]
• 이금화 [ GumHwa Lee | 조선대학교 약학대학 ]
• 신현재 [ Hyun-Jae Shin | 조선대학교 생명화학고분자공학과 ] Corresponding author

참고문헌