Earticle

초록

영어

In this study, we analyze the antioxidant and anti-inflammatory activities of various extracts of Cryptoporus volvatus. Three extracts were prepared using hot-water, 70% ethanol, and 70% methanol. Compared to the hot-water and 70% methanol extracts, the 70% C. volvatus ethanol extracts showed significantly higher DPPH radical scavenging activities (76.1%), nitrite scavenging activities (29.5%), and total polyphenol content (9.17 mg GAE/g). The DPPH radical and nitrite scavenging activities of C. volvatus both correlated significantly with the total polyphenol content. The Pearson’s correlation coefficient of DPPH radical and nitrite scavenging activities with total polyphenol content were r = 0.744 (p<0.05) and r = 0.921 (p<0.01), respectively. To test their anti-inflammatory effects, nitric oxide production and cell viability were measured by performing nitric oxide (NO) and MTT assays on lipopolysaccharide-treated RAW 264.7 cells. The NO production levels of the 70% ethanol extract-treated cells were slightly lower than those of the others. In our MTT assay, the hot-water extracts showed higher cell viabilities (81.4%) than those of 70% ethanol and 70% methanol extracts. The ?-glucan content of C. volvatus was 37.8%. From the results of this study, it is suggested that C. volvatus, an easily accessible wild mushroom, has potential to be used in the development of medicines, cosmetics, and functional health foods.

목차

ABSTRACT
서 론
재료 및 방법
공시시료
추출용매별 분석용 시료 제조
DPPH 라디컬 소거능(DPPH radical-scavenging activity)
아질산염 소거능(Nitrite-scavenging activity)
총 폴리페놀 함량(Total polyphenol contents)
베타글루칸 함량분석(β-glucan contents)
세포배양
Nitric oxide (NO) 생성률 측정
세포생존율 측정(MTT assay)
통계처리
결과 및 고찰
DPPH 라디컬 소거능(DPPH radical-scavenging activity)
아질산염 소거능(Nitrite-scavenging activity)
총 폴리페놀 함량(Total polyphenol contents)
한입버섯 추출물의 Nitric Oxide (NO) 생성률
한입버섯 추출물이 세포생존율에 미치는 영향
한입버섯의 베타글루칸 함량
적 요
REFERENCES

저자

• 안기홍 [ Gi-Hong An | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 한재구 [ Jae-Gu Han | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 조재한 [ Jae-Han Cho | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ] Corresponding Author

참고문헌