Earticle

초록

영어

This study was carried out to analyze the physiological activities of wild mushroom extracts collected from the Gangwon-do, Gyeonggi-do, and Chungcheongbuk-do provinces in Korea. Among the wild mushroom extracts, those of Clitocybe robusta and Leucopaxillus giganteus (OK829) showed the highest DPPH radical scavenging activities. Nitrite scavenging activity of the L. giganteus extract (OK811) was determined to be 64.1%, which is considerably higher than those of the other mushroom extracts analyzed in this study. The total polyphenol levels in Suillus granulatus, L. giganteus (OK829), and Amanita manginiana extracts were found to be 19.7 mg GAE/g, 20.2 mg GAE/g, and 22.3 mg GAE/g, respectively. To determine their antiinflammatory effects, nitric oxide production, and cell viability, NO measurement and MTT assays were performed using lipopolysaccharide (LPS)-treated RAW 264.7 cells. The levels of nitric oxide (NO) produced by the C. robusta and Hypholoma fasciculare extracts were remarkably lower than those produced by the others. In our MTT assay, the extracts of S. granulatus, L. giganteus (OK811), and Lactarius chrysorrheus showed high cell viabilities of 40.3%, 48.3%, and 43.2%, respectively. These results can provide the fundamental data for extracting useful compounds from wild mushrooms.

목차

ABSTRACT
서론
재료 및 방법
국내 야생버섯 수집
추출용매별 분석용 시료 제조
DPPH 라디컬 소거능(DPPH radical-scavenging activity)
아질산염 소거능(Nitrite-scavenging activity)
총 폴리페놀 함량(Total polyphenol contents)
세포배양
NO 저해활성 측정(NO assay)
세포생존율 측정(MTT assay)
통계처리
결과 및 고찰
야생수집 버섯추출물의 DPPH 라디컬 소거능
야생수집 버섯추출물의 아질산염 소거능
야생수집 버섯추출물의 총 폴리페놀 함량
야생수집 버섯추출물의 NO 생성 저해도
야생수집 버섯추출물이 세포생존율에 미치는 영향
적요
감사의 글
REFERENCES

저자

• 안기홍 [ Gi-Hong An | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 조재한 [ Jae-Han Cho | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 이강효 [ Kang-Hyo Lee | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ]
• 한재구 [ Jae-Gu Han | 농촌진흥청 국립원예특작과학원 인삼특작부 버섯과 ] Corresponding Author

참고문헌