Earticle

초록

영어

The objective of this study was to evaluate antioxidant effect and tyrosinase inhibitory activity of white beech mushroom (Hypsizygus marmoreus) extracts. The white beech mushroom was extracted into hot water and methanol. Total polyphenol content was highest in the hot water extract (8.4±3.27 mg GAE/g) compared to the methanol extract (7.3±2.85 mg GAE/g). The flavonoids contents in hot water and methanol extracts were 3.8±3.81 ug/mg and 2.5±1.95 ug/mg, respectively. The tyrosinase inhibitory activity of extract was increased in a dose dependent manner and tyrosinase inhibitory activity of extract (hot water extract, 69.72%; methanol extract, 52.67% at 40 mg/ml) was lower than those of positive control 2% arbutin (96%). The DPPH radical scavenging activity of the hot water and methanol extract was 80% and 74%, respectively. Hot water extract (63.34±1.00 uM TE/g) were more effective in ORAC (oxygen radical absorbance capacity) value than methanol extract (46.33±0.48 uM TE/g). The toxicity of hot water and methanol extracts was investigated using WST-1 (4-[3-(4-iodophenyl)-2-(4- nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulphonate) assay on the B16BL6 melanoma cells.

목차

ABSTRACT
서론
재료 및 방법
흰색 느티만가닥버섯 추출물의 제조
총 폴리페놀 함량 측정
총 플라보노이드 함량 측정
Tyrosinase 저해 활성
DPPH 라디칼소거 활성
Oxygen radical absorbance capacity (ORAC) 측정
세포 생존율 측정
통계처리
결과 및 고찰
흰색 느티만가닥버섯 추출물의 총 폴리페놀 및 플라보노이드 함량
흰색 느티만가닥버섯 추출물의 tyrosinase 저해 활성
흰색 느티만가닥버섯 추출물의 DPPH 라디칼소거 활성
흰색 느티만가닥버섯 추출물의 Oxygen radical absorbance capacity (ORAC)
흰색 느티만가닥버섯 추출물이 B16BL6 melanoma cell에 미치는 영향
적요
REFERENCES

저자

• 김수철 [ Su Cheol Kim | 경남과학기술대학교 제약공학과 ]
• 김혜수 [ Hye Soo Kim | 경남과학기술대학교 제약공학과 ]
• 조수정 [ Soo Jeong Cho | 경남과학기술대학교 제약공학과 ] Corresponding Author

참고문헌