Earticle

초록

영어

The purpose of this study is to discover a food material having anti-obesity effects and to disseminate information on the effects of the material to people who are interested in anti-obesity. For this study, 11 kinds of Auricularia (wood ear) spp., including 8 strains of Auricularia auricula-polytricha, and 3 strains of A. auricula-judae, were presented by the Jeollanamdo Agricultural Research & Extension Services. 3T3-L1 (preadipocyte cell) was used for identifying the inhibition effect on adipocyte differentiation. As a result, this study found that all the extracts had slightly different degrees of inhibition effects on adipocyte differentiation. Among the A. auricula-polytricha strains, strain 21001 showed the most significant effect (4.58%), and the inhibition effect of strain 21002 (4.43%) was the greatest among A. auricula-judae strains. Overall, the inhibition effect of A. auricula-polytricha strains was greater than that of A. auricula-judae strains. The results of mRNA and protein analysis also demonstrated that the inhibition effect of A. auricula-polytricha 21001 was superior to that of any other strains. An in vivo study using 56 ICR mice (6w, male), was performed for 4 weeks. A. auricula-polytricha 21001, which exhibited the most significant effect in the in vitro study was used to compose six different types of feeds. Daily body weight gain of the high-fat diet containing 0.2% 21001 extract feeding group was 0.22±0.08 g (*p < 0.05), and it was 31.25% lower than that of the high-fat diet feeding group (0.32±0.06). Internal organ weight measurement and blood analysis were performed immediately after animal sacrifice. The results proved that treatment with more than 0.1% of A. auricula-polytricha strain 21001 could significantly reduce (more than *p < 0.05) the weight of liver and epididymal fat, and levels of glucose, total cholesterol, AST, and ALT in blood.

목차

ABSTRACT
서론
재료 및 방법
버섯 시료의 제조
지방세포 분화 억제 시험
동물실험
결과 및 고찰
세포생존율
목이버섯 추출물의 지방세포 분화 억제 효과
발현량
Western blot을 이용한 지방세포 분화 단백질 마커 발현 조사
동물실험
적요
감사의 글
REFERENCES

저자

• 박건희 [ Kun Hee Park | 성균관대학교 식품생명공학과 ]
• 김길자 [ Kil-ja Kim | 전라남도 농업기술원 식량작물연구소 ]
• 장갑열 [ Kab Yeul Jang | 농촌진흥청 국립원예특작과학원 버섯과 ]
• 박기문 [ Kimoon Park | 성균관대학교 식품생명공학과 ] Corresponding author

참고문헌