Earticle

초록

영어

This study was performed to determine optimal extraction conditions of fermented Polygonum multiflorum root by Lentinula edodes (JMI10079) Pegler mycelials using response surface methodology. The independent factors were extraction temperature (X1: 40–100oC), extraction time (X2: 2–10 hrs.), and the ratio of water to sample (X3: 33–100 mg/mL). Their effects were assessed on dependent variables of the extract properties, which included soluble solid contents (Y1), °Brix of sample extract (Y2), total polyphenol content (Y3), total flavonoid content (Y4), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical cation scavenging activity (Y5) and 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (Y6). The experimental data obtained were fit to a second-order polynomial equation. The optimal extraction conditions for fermented P. multiflorum root were: X1: 91.22°C, X2: 7.72 hrs, and X3: 39.71 mg/mL.

목차

ABSTRACT
 서론
 재료 및 방법
  재료
  시약
  반응표면 분석 시험 설계
  고형분 함량
  당도 측정
  Total polyphenol 함량 측정
  Total flavonoid 함량 측정
  ABTS cation radical scavenging activity
  DPPH radical scavenging activity
  통계분석
 결과 및 고찰
  추출물의 특성
  고형분 함량
  당도 측정
  Total polyphenol 함량 및 Total flavonoid 함량
  항산화 활성
  열수 추출조건의 최적화
 요약
 감사의 글
 References

저자

• 오준석 [ Junseok Oh1 | 동부생약영농조합법인 ]
• 홍재희 [ Jae-Heoi Hong | 동부생약영농조합법인 ]
• 박태영 [ Tae-Young Park | 동부생약영농조합법인 ]
• 신지은 [ Ji-eun Shin | 충남대학교 정보통계학과 ]
• 김경제 [ Kyung-Je Kim | (재)장흥군버섯산업연구원 ]
• 진성우 [ Seong-Woo Jin | (재)장흥군버섯산업연구원 ]
• 반승언 [ Seung-Eon Ban | (재)장흥군버섯산업연구원 ]
• 고영우 [ Young-Woo Koh | (재)장흥군버섯산업연구원 ]
• 임승빈 [ Seung-Bin Im | (재)장흥군버섯산업연구원 ]
• 서경순 [ Kyoung-Sun Seo | (재)장흥군버섯산업연구원 ] Corresponding author

참고문헌