Earticle

초록

영어

Oyster mushroom is a type of mushroom that is commonly cultivated and consumed in Korea. P. ostreatus ‘Suhan’ is a preferred cultivar for many mushroom farmers because it has a dark pileus and thick stipe. However, as it is very sensitive to environmental conditions, farmers consistently demand an alternative cultivar. To develop a new cultivar, the parental strains KMCC01680 (‘Suhan’) and KMCC00478 (‘Gosol’) were selected from various collected P. ostreatus strains by cultivating genetic resources. P. ostreatus ‘Heuksol’ was developed by the method of Mon-Mon crossing between monokaryotic strains derived from ‘Suhan’ and ‘Gosol’. Thirty strains of 174 crossed strains were initially selected by cultivation experiments. After bulk cultivation tests, ‘Heuksol’ was selected. The nuclear DNA profile of ‘Heuksol’ was similar to those of the parental strains, ‘Suhan’ and ‘Gosol’, when RAPD (random amplified polymorphic DNA) primers and UPF (Universal PCR Fingerprinting) 2, 3, and 4 were used. The optimum temperature for mycelial growth was 30oC for ‘Heuksol’, but medium-high temperatures were also appropriate, especially 13–20oC. The fruiting body production per bottle (1,100 mL) was approximately 140.8 g. When compared to the control strain ‘Suhan’, the thickness of the stipe of ‘Heuksol’ was greater than that of ‘Suhan’ (13.5 mm vs 9.4 mm). The pileus diameter of ‘Heuksol’ was similar to that of ‘Suhan’ and the pileus thickness of ‘Heuksol’ and ‘Suhan’ was 19.7 mm and 21.8 mm, respectively. ‘Heuksol’ had more a productive stipe number than ‘Suhan’ and the pileus of ‘Heuksol’ was dark gray, even at high temperatures. Therefore, it was suggested that this new cultivar, ‘Heuksol’, could provide an alternative to ‘Suhan’ and contribute to the profit of oyster mushroom farms.

목차

ABSTRACT
 서론
 재료 및 방법
  균주 및 배양
  단포자 분리 및 교배
  교잡 및 교잡계통 자실체 특성 검정
  DNA 다형성 분석
 결과 및 고찰
  육성경위
  고유특성
  가변특성
  DNA 다형성 분석
  자실체 수량성 및 재배상 유의점
 적요
 감사의 말씀
 References

저자

• 오민지 [ Min-Ji Oh | 국립원예특작과학원 버섯과 ]
• 임지훈 [ Ji-Hoon Im | 국립원예특작과학원 버섯과 ]
• 신평균 [ Pyung-Gyun Shin | 국립원예특작과학원 버섯과 ]
• 오연이 [ Youn-Lee Oh | 국립원예특작과학원 버섯과 ]
• 장갑열 [ Kab-Yeul Jang | 국립원예특작과학원 버섯과 ]
• 공원식 [ Won-Sik Kong | 국립원예특작과학원 버섯과 ] Corresponding author

참고문헌